[Relationship between propionate metabolism and TCA cycle in rumen epithelium of normal starved and alloxan diabetic sheep].

It was reported that there was a relationship between the propionate metabolism and TCA cycle, and that starvation and alloxan treatment influenced the propionate metabolism, and the relationships between the propionate metabolism and glucolysis were changed by the alloxan treatment in the rumen epithelium of sheep. Further study was designed to elucidiate the relationship betweenthe propionate metabolism and TCA cycle, and how this relationship was influenced by the starvation and alloxan treatments. The following results were obtained in a study on influence of citrate or succinate on the "C-propionate metabolism and of propionate on the metabolism of "C-citrate or -succinate in the rumen epithelium of normally fed, starved and alloxan diabetic sheep. In the rumen epithelium of normally fed and starvedsheep, the 14CO2 formation from 14,,upropionate was increased and the transfer of "C from "C-propionate into glucose, triglyceride and phospholipid was decreased by the addition of citrate or succinate. And also the formation of 14CO2, 14C-triglyceride and -phospholipid from '4C-citrate or -succinate was increased and the transfer of '4C from '4C-citrate or -succinate into glucose , ketone bodies and cholesterol was decreased by the propionate addition in the rumen epithelium of normally fed and starved animals. Furthermore, the citrate effects on the propionate metabolism and the propionate effects on the citrate metabolism were disappeared by the alloxan treatment. But the succinate effects on the propionate metabolism and the propionate effects on the succinate metabolism were observed in the rumen epithelium ofalloxan diabetic animals. As mentioned above, the citrate or succinate showedthe various effects on the propionate metabolism and also the propionate had various effects on the metabolism of citrate and succinate, and these relationships between the propionate metabolism and the citrate metabolism were changed by alloxan treatment in the rumen epithelium of sheep. (See pp. 67,-,-73)